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cell surface sialic acid  (Vector Laboratories)


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    Structured Review

    Vector Laboratories cell surface sialic acid
    <t>Cell</t> <t>surface</t> <t>sialic</t> <t>acid</t> levels on PI cells are similar to naïve cells. ( A , B ) Naïve and PI H1975 cells were plated in a 24-well plate. Naïve cells were mock infected as a negative control or acutely infected with PIV5 at an MOI of 10 PFU/cell. The cells were then stained with an anti-PIV5 HN monoclonal antibody and processed via flow cytometry. ( C ) Mock-infected, AI, and PI cells were treated with 1 U/mL Clostridium perfringens neuraminidase (NA) immediately following infection. At 24 hpi, cells were stained with the lectin MAL II followed by conjugated streptavidin, then analyzed via flow cytometry. MFI = mean fluorescence intensity. ( D ) Mock-infected, AI and PI cells were treated with NA immediately following infection. At 24 hpi, cells were left untreated, treated with 10% HI NHS, or treated with 10% NHS. Red fluorescent images were captured and quantified using the IncuCyte as described in . *** and **** show p -values of <0.001 and <0.0001.
    Cell Surface Sialic Acid, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+surface+sialic+acid/N-acetylneuraminic+acid+(sialic+acid)/pmc12388906-89-0-21
    Average 94 stars, based on 258 article reviews
    cell surface sialic acid - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis"

    Article Title: Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis

    Journal: Pathogens

    doi: 10.3390/pathogens14080815

    Cell surface sialic acid levels on PI cells are similar to naïve cells. ( A , B ) Naïve and PI H1975 cells were plated in a 24-well plate. Naïve cells were mock infected as a negative control or acutely infected with PIV5 at an MOI of 10 PFU/cell. The cells were then stained with an anti-PIV5 HN monoclonal antibody and processed via flow cytometry. ( C ) Mock-infected, AI, and PI cells were treated with 1 U/mL Clostridium perfringens neuraminidase (NA) immediately following infection. At 24 hpi, cells were stained with the lectin MAL II followed by conjugated streptavidin, then analyzed via flow cytometry. MFI = mean fluorescence intensity. ( D ) Mock-infected, AI and PI cells were treated with NA immediately following infection. At 24 hpi, cells were left untreated, treated with 10% HI NHS, or treated with 10% NHS. Red fluorescent images were captured and quantified using the IncuCyte as described in . *** and **** show p -values of <0.001 and <0.0001.
    Figure Legend Snippet: Cell surface sialic acid levels on PI cells are similar to naïve cells. ( A , B ) Naïve and PI H1975 cells were plated in a 24-well plate. Naïve cells were mock infected as a negative control or acutely infected with PIV5 at an MOI of 10 PFU/cell. The cells were then stained with an anti-PIV5 HN monoclonal antibody and processed via flow cytometry. ( C ) Mock-infected, AI, and PI cells were treated with 1 U/mL Clostridium perfringens neuraminidase (NA) immediately following infection. At 24 hpi, cells were stained with the lectin MAL II followed by conjugated streptavidin, then analyzed via flow cytometry. MFI = mean fluorescence intensity. ( D ) Mock-infected, AI and PI cells were treated with NA immediately following infection. At 24 hpi, cells were left untreated, treated with 10% HI NHS, or treated with 10% NHS. Red fluorescent images were captured and quantified using the IncuCyte as described in . *** and **** show p -values of <0.001 and <0.0001.

    Techniques Used: Infection, Negative Control, Staining, Flow Cytometry, Fluorescence

    Related Articles

    Flow Cytometry:

    Article Title: Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis
    Article Snippet: C3 and MAC surface deposition were monitored using an anti-C3 antibody (1:1000, 204869, Calbiochem, San Diego, CA, USA), or anti-SC5b-9 antibody (1:500, A239, Quidel, San Diego, CA, USA), followed by antibody staining using AlexaFluor405 antibodies specific for the individual species of antibody. .. Cell surface sialic acid was monitored by flow cytometry using MALL II Maackia Amurensis Lectin II that was biotinylated (1:50, B-1265-1, Vector Laboratories, Newark, CA, USA) and then with treatment with Pacific Blue conjugated Streptavidin (1:1000, S11222 , Thermo Fisher Scientific, Waltham, MA, USA). ..



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    Image Search Results


    Cell surface sialic acid levels on PI cells are similar to naïve cells. ( A , B ) Naïve and PI H1975 cells were plated in a 24-well plate. Naïve cells were mock infected as a negative control or acutely infected with PIV5 at an MOI of 10 PFU/cell. The cells were then stained with an anti-PIV5 HN monoclonal antibody and processed via flow cytometry. ( C ) Mock-infected, AI, and PI cells were treated with 1 U/mL Clostridium perfringens neuraminidase (NA) immediately following infection. At 24 hpi, cells were stained with the lectin MAL II followed by conjugated streptavidin, then analyzed via flow cytometry. MFI = mean fluorescence intensity. ( D ) Mock-infected, AI and PI cells were treated with NA immediately following infection. At 24 hpi, cells were left untreated, treated with 10% HI NHS, or treated with 10% NHS. Red fluorescent images were captured and quantified using the IncuCyte as described in . *** and **** show p -values of <0.001 and <0.0001.

    Journal: Pathogens

    Article Title: Relationship Between Cell Surface Viral Glycoprotein Expression and Resistance of Parainfluenza Virus Persistently Infected Cells to Complement-Mediated Lysis

    doi: 10.3390/pathogens14080815

    Figure Lengend Snippet: Cell surface sialic acid levels on PI cells are similar to naïve cells. ( A , B ) Naïve and PI H1975 cells were plated in a 24-well plate. Naïve cells were mock infected as a negative control or acutely infected with PIV5 at an MOI of 10 PFU/cell. The cells were then stained with an anti-PIV5 HN monoclonal antibody and processed via flow cytometry. ( C ) Mock-infected, AI, and PI cells were treated with 1 U/mL Clostridium perfringens neuraminidase (NA) immediately following infection. At 24 hpi, cells were stained with the lectin MAL II followed by conjugated streptavidin, then analyzed via flow cytometry. MFI = mean fluorescence intensity. ( D ) Mock-infected, AI and PI cells were treated with NA immediately following infection. At 24 hpi, cells were left untreated, treated with 10% HI NHS, or treated with 10% NHS. Red fluorescent images were captured and quantified using the IncuCyte as described in . *** and **** show p -values of <0.001 and <0.0001.

    Article Snippet: Cell surface sialic acid was monitored by flow cytometry using MALL II Maackia Amurensis Lectin II that was biotinylated (1:50, B-1265-1, Vector Laboratories, Newark, CA, USA) and then with treatment with Pacific Blue conjugated Streptavidin (1:1000, S11222 , Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Infection, Negative Control, Staining, Flow Cytometry, Fluorescence